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Figure 4. The effect of Mosla japonica extract (MJE) on the expression of phosphorylated ERK (P-ERK), JNK (P-JNK), and p38 (P-p38) proteins in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells. Cells were treated with MJE (25, 50, and 100 µg/mL) and LPS (1 µg/mL) for 20 min. MAPK <t>inhibitors—PD98059</t> for ERK, SP600125 for JNK, and SB203580 for p38—were used as controls. (a,c,e) Western blot analysis of P-ERK, P-JNK, and P-p38 protein expression. (b,d,f) Quantification of P-ERK, P-JNK, and P-p38 protein levels. Data are presented as mean ± SD from three independent experiments. Statistical significance: # p < 0.001 vs. unstimulated control, * p < 0.05, *** p < 0.001 vs. LPS-only group.
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Figure 4. The effect of Mosla japonica extract (MJE) on the expression of phosphorylated ERK (P-ERK), JNK (P-JNK), and p38 (P-p38) proteins in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells. Cells were treated with MJE (25, 50, and 100 µg/mL) and LPS (1 µg/mL) for 20 min. MAPK <t>inhibitors—PD98059</t> for ERK, SP600125 for JNK, and SB203580 for p38—were used as controls. (a,c,e) Western blot analysis of P-ERK, P-JNK, and P-p38 protein expression. (b,d,f) Quantification of P-ERK, P-JNK, and P-p38 protein levels. Data are presented as mean ± SD from three independent experiments. Statistical significance: # p < 0.001 vs. unstimulated control, * p < 0.05, *** p < 0.001 vs. LPS-only group.
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Figure 4. The effect of Mosla japonica extract (MJE) on the expression of phosphorylated ERK (P-ERK), JNK (P-JNK), and p38 (P-p38) proteins in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells. Cells were treated with MJE (25, 50, and 100 µg/mL) and LPS (1 µg/mL) for 20 min. MAPK inhibitors—PD98059 for ERK, SP600125 for JNK, and SB203580 for p38—were used as controls. (a,c,e) Western blot analysis of P-ERK, P-JNK, and P-p38 protein expression. (b,d,f) Quantification of P-ERK, P-JNK, and P-p38 protein levels. Data are presented as mean ± SD from three independent experiments. Statistical significance: # p < 0.001 vs. unstimulated control, * p < 0.05, *** p < 0.001 vs. LPS-only group.

Journal: Life

Article Title: Anti-Inflammatory Effects and Human Skin Safety of the Eastern Traditional Herb Mosla japonica

doi: 10.3390/life15030418

Figure Lengend Snippet: Figure 4. The effect of Mosla japonica extract (MJE) on the expression of phosphorylated ERK (P-ERK), JNK (P-JNK), and p38 (P-p38) proteins in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells. Cells were treated with MJE (25, 50, and 100 µg/mL) and LPS (1 µg/mL) for 20 min. MAPK inhibitors—PD98059 for ERK, SP600125 for JNK, and SB203580 for p38—were used as controls. (a,c,e) Western blot analysis of P-ERK, P-JNK, and P-p38 protein expression. (b,d,f) Quantification of P-ERK, P-JNK, and P-p38 protein levels. Data are presented as mean ± SD from three independent experiments. Statistical significance: # p < 0.001 vs. unstimulated control, * p < 0.05, *** p < 0.001 vs. LPS-only group.

Article Snippet: The MAPK inhibitors SB203580 (sc-3533, p38 MAPK inhibitor), Life 2025, 15, 418 3 of 16 SP600125 (sc-200635, JNK inhibitor), and PD98059 (sc-3532, MEK1/2 inhibitor targeting MAPK/ERK pathway) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Expressing, Western Blot, Control